A novel screening method for ingredients of essential oil to identify antibacterial compounds based on growth curve shape

We established a novel screening method to identify antibacterial compounds and used the screen various triterpenoids, which are difficult assay using existing methods. The involves comparison of shape bacterial growth curves based on changes in culture turbidity. Growth were grouped multiple classification analysis. tested belonged different chemical group than controls. Six four triterpenoids screened suppressed Staphylococcus aureus FDA209P Escherichia coli. W3110, respectively. Two exhibited bactericidal activity against S. aureus, others bacteriostatic activity.